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Simple and sensitive high‐performance liquid chromatography method for the quantitation of indinavir in rat plasma and central nervous system
Author(s) -
Hamidi Mehrdad
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500328
Subject(s) - chromatography , indinavir , high performance liquid chromatography , chemistry , central nervous system , plasma , simple (philosophy) , human immunodeficiency virus (hiv) , medicine , sida , immunology , physics , viral disease , philosophy , epistemology , quantum mechanics
Abstract A simple and sensitive RP‐HPLC method using UV detection (215 nm) was developed for the determination of indinavir concentrations in rat plasma, cerebrospinal fluid (CSF), and brain tissue homogenates. Biological samples were processed using a combination of acid pretreatment and liquid–liquid extraction with verapamil used as the internal standard. This method produced a linear response throughout the indinavir concentration range of 0.05–30 μM in plasma and 0.05–2.5 μM in CSF and brain with a LOD of 12.5 nM for plasma and CSF, and 6.25 nM for brain homogenate. Due to its high sensitivity, this assay is particularly useful for the quantitative determination of indinavir concentrations in brain and CSF.