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A preparative isolation and purification of arctigenin and matairesinol from Forsythia koreana by centrifugal partition chromatography
Author(s) -
Kim Chul Young,
Ahn MiJeong,
Kim Jinwoong
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500316
Subject(s) - chromatography , chemistry , forsythia , hexane , ethyl acetate , pinoresinol , methanol , high performance liquid chromatography , solvent , lignan , organic chemistry , medicine , honeysuckle , alternative medicine , pathology , traditional chinese medicine
Abstract Centrifugal partition chromatography was applied to separate arctigenin and matairesinol from Forsythia koreana extract with a two‐phase solvent system composed of n ‐hexane–ethyl acetate–methanol–water (5 : 5 : 5 : 5 v/v). Using this method, arctigenin and matairesinol were successfully separated from partially purified F. koreana extracts in only one step. The purities of isolated compounds were determined to be over 90% by HPLC analysis.