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Determination of 19 rhubarb constituents by high‐performance liquid chromatography–ultraviolet–mass spectrometry
Author(s) -
Lin ChingChe,
Wu ChuanI,
Lin TaChen,
Sheu ShuennJyi
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500307
Subject(s) - chemistry , chromatography , anthraquinones , mass spectrometry , absorbance , acetic acid , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , botany , biology
Rhubarb ( Rhei rhizoma ), a commonly used Chinese herb, contains anthraquinones, anthrones, galloylglucoses, stilbenes, and flavan‐3‐ols compounds, etc. as major constituents. Using 19 of these compounds as markers, an HPLC‐UV‐MS method was developed to estimate the quality of rhubarb samples within a period of 70 min. Extracts were analyzed with a Cosmosil 5C 18 ‐MS column and eluted with a gradient comprising an aqueous solution of acetic acid and methanol at a flow rate of 0.9 mL/min. Peaks were detected by absorbance measurements at 254 nm ( 6 and 8 – 19 ) and 280 nm ( 1 – 5 and 7 ), and the peaks of the marker substances were identified from their UV spectra and MS fragmentation patterns. The proposed method yielded a peak–area ratio RSD value with an intraday SD falling within 0.71–1.78% and an interday SD within 0.78–1.98% at a detection limit of 0.2–3.2 μg/mL. The ESI negative ion mode was used to collect data (molecular weight, CID fragments from MS and MS/MS spectra) for 19 compounds from four types of structure categories: anthraquinones, dianthrone glycosides, stilbenes, and galloylglucosides. The information gathered can be used to identify the structures of various peaks appearing in the LC chromatograms of rhubarb samples.