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Optimization of the solid‐phase microextraction method in the determination of Ixodes ricinus (L.) volatiles
Author(s) -
Zahradníčková Helena,
Bouman Edwin A. P.
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500282
Subject(s) - chromatography , solid phase microextraction , chemistry , acetophenone , extraction (chemistry) , ixodes ricinus , ricinus , solid phase extraction , analytical chemistry (journal) , gas chromatography–mass spectrometry , mass spectrometry , tick , organic chemistry , biochemistry , catalysis , ecology , biology
For the first time, headspace solid‐phase microextraction coupled with GC‐MS analysis was used to study volatile compounds emitted by the tick Ixodes ricinus (L.). Variables such as the type of SPME fibre, equilibration time and extraction time have been evaluated with the mixture of four selected standards, a so‐called artificial tick (acetophenone, racemic 4‐heptanolide, methyl 2‐methoxybenzoate and methyl 3‐chloro‐4‐methoxybenzoate). Optimized conditions were obtained by the use of polydimethylsiloxan 100 μm fibre at 30 min equilibration and 15 min extraction time. The method proved to have a good linearity ( r 2 >0.98) at a concentration range from 0.5 ng (1 ng for methyl 3‐chloro‐4‐methoxybenzoate) to 25 ng. LODs for a compound ranged between 0.19 and 1 ng, RSD (%) ranged from 13.76 to 25.08. The determination of 1.99 ng of methyl 3‐chloro‐4‐methoxybenzoate emitted by five engorged females proved the usefulness of the developed method to identify and quantify the volatile compounds emitted by I. ricinus ticks.