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Validation of a novel HPLC sorbent material for the determination of ten quinolones in human and veterinary pharmaceutical formulations
Author(s) -
Samanidou Victoria F.,
Christodoulou Eleni A.,
Papadoyannis Ioannis N.
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500262
Subject(s) - chromatography , high performance liquid chromatography , sorbent , chemistry , adsorption , organic chemistry
A novel sorbent material of ultrapure silica gel provided with novel State of the Art Bonding‐ and Endcapping Technology commercially available under the name PerfectSil® Target (250×4 mm, ODS‐3, 5 μm, by MZ‐Analysentechnik, Germany) was used and validated for the sensitive HPLC determination of ten quinolone antibiotics: enoxacin, ofloxacin, norfloxacin, ciprofloxacin, danofloxacin (DAN), enrofloxacin (ENR), sarafloxacin, oxolinic acid (OXO), nalidixic acid (NAL), and flumequine. The analytical column validation was performed in terms of separation efficiency, precision, and peak asymmetry. The separation was achieved at ambient temperature using a mobile phase of TFA (0.1%)–CH 3 OH–CH 3 CN delivered under the optimum gradient program, at a flow rate of 1.2 mL/min. Photodiode array detection was used and eluant was monitored at 275 nm. For the quantitative determination caffeine (7.5 ng/μL) was used as internal standard. The achieved LODs were 0.03 ng/μL per 50 μL injected volume for OXO, 0.1 ng/μL for DAN, ENR, and NAL, and 0.2 ng/μL for the remaining six studied quinolones. The method was validated in terms of interday ( n = 6) and intraday ( n = 5) precision and accuracy. The proposed method was successfully applied to the analysis of pharmaceutical formulations destined either for human or for veterinary use.