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Extending the array of crosslinkers suitable for the preparation of polymethacrylate‐based monoliths
Author(s) -
Nordborg Anna,
Svec Frantisek,
Fréchet Jean M. J.,
Irgum Knut
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500222
Subject(s) - ethylene glycol dimethacrylate , triethylene glycol , methacrylate , monolith , polymerization , diethylene glycol , chemistry , azobisisobutyronitrile , ethylene glycol , chromatography , polyurethane , pentaerythritol , copolymer , polymer chemistry , materials science , methacrylic acid , organic chemistry , polymer , fire retardant , catalysis
The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 μm ID capillaries and their application for μ‐HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1‐propanol and 1,4‐butanediol yielded monoliths with pore sizes above 1 μm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.