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Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin‐B in Artemisia annua using reversed‐phase thin layer chromatography
Author(s) -
Bhandari Pamita,
Gupta Ajai P.,
Singh Bikram,
Kaul Vijay K.
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500198
Subject(s) - chemistry , chromatography , artemisia annua , derivatization , artemisinin , thin layer chromatography , reagent , sesquiterpene , high performance thin layer chromatography , high performance liquid chromatography , stereochemistry , organic chemistry , plasmodium falciparum , malaria , immunology , biology
A rapid and simple RP‐TLC method for simultaneous quantification of pharmacologically important sesquiterpene artemisinin (AM) together with its precursors arteannuin‐B (AB) and artemisinic acid (AA) in the inflorescence part of Artemisia annua plant has been developed. The RP‐TLC of sesquiterpenes was performed on RP‐18 F 254 S thin‐layer chromatographic plates by developing in mobile phase, containing 0.2% TFA in water/ACN (35 : 65, v/v). The densitometric determination of AM, AB and AA was carried out after derivatization with anisaldehyde reagent at 426 nm in absorption‐reflectance mode.