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Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD
Author(s) -
Jaime Laura,
Mendiola José A.,
Herrero Miguel,
SolerRivas Cristina,
Santoyo Susana,
Señorans F. Javier,
Cifuentes Alejandro,
Ibáñez Elena
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500185
Subject(s) - spirulina (dietary supplement) , chromatography , extraction (chemistry) , high performance liquid chromatography , chemistry , dpph , antioxidant , chromatography detector , solvent , organic chemistry , raw material
A new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) and different chromatographic procedures, such as TLC, at preparative scale, and HPLC with a diode array detector (DAD). Different solvents were tested for PLE extraction of antioxidants from S. platensis microalga. An optimized PLE process using ethanol (generally recognized as safe, GRAS) as extraction solvent has been obtained that provides natural extracts with high yields and good antioxidant properties. TLC analysis of this ethanolic extract obtained at 115°C for 15 min was carried out and the silica layer was stained with a DPPH∗ (diphenyl‐pycril‐hydrazyl) radical solution to determine the antioxidant activity of different chromatographic bands. Next, these colored bands were collected for their subsequent analysis by HPLC‐DAD, revealing that the compounds with the most important antioxidant activity present in Spirulina extracts were carotenoids, as well as phenolic compounds and degradation products of chlorophylls.