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Simultaneous determination of carotenoids, tocopherols, and γ‐oryzanol in crude rice bran oil by liquid chromatography coupled to diode array and mass spectrometric detection employing silica C30 stationary phases
Author(s) -
Stöggl Wolfgang,
Huck Christain,
Wongyai Surapote,
Scherz Heimo,
Bonn Günther
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500176
Subject(s) - chromatography , chemistry , rice bran oil , high performance liquid chromatography , carotenoid , bran , organic chemistry , raw material , food science
Crude rice bran oil contains tocopherols (vitamin E), carotenoids (vitamin A), and phytosterols, which possess antioxidant activities and show promising effects as preventive and therapeutic agents. The aim of this work was to establish methods and to compare C18 and C30 silica stationary phases in order to separate and detect tocopherols, carotenoids, and γ‐oryzanol in one single run. Comparing RP‐LC on silica C18 and C30, higher resolution between all target compounds was obtained using the C30 stationary phase. Methanol was used as eluent and the elution strength was increased by the addition of tert ‐butyl methyl ether for highly hydrophobic analytes such as γ‐oryzanol. Detection was accomplished by diode array detection from 200 to 500 nm. Absorbance maxima were found at 295 nm for tocopherols, 324 nm for γ‐oryzanol, and 450 nm for carotenoids. Furthermore, compounds were characterized and identified on the basis of their UV‐spectra. Both RP systems were coupled to MS (LC‐MS) by using an atmospheric pressure chemical ionization interface.