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Silica monolithic columns: Synthesis, characterisation and applications to the analysis of biological molecules
Author(s) -
Rieux Laurent,
Niederländer Harm,
Verpoorte Elisabeth,
Bischoff Rainer
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500146
Subject(s) - monomer , mesoporous silica , monolithic hplc column , mesoporous material , high pressure , silicon dioxide , materials science , mass spectrometry , pressure drop , chemistry , nanotechnology , chromatography , chemical engineering , polymer , high performance liquid chromatography , organic chemistry , catalysis , thermodynamics , physics , engineering physics , engineering , metallurgy
Abstract In recent years, proteomics has been a subject of intense research. The complexity of proteomics samples has fostered technological developments. One of these addresses the need for more efficient and faster separations. Monolithic columns prepared from organic and silica monomers offer very efficient separations at low backpressure. Silica‐based monoliths have small‐sized skeletons and a bimodal pore size distribution with μm‐sized throughpores and nm‐sized mesopores. This gives silica‐based monoliths favourable properties for high‐efficiency, fast separations, like a low‐pressure drop across the column, fast mass transfer kinetics and a high binding capacity.