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Separation and determination of anthraquinones in Cassia obtusifolia (Leguminosae) by micellar electrokinetic capillary electrophoresis
Author(s) -
Jiang TingFu,
Lv ZhiHua,
Wang YuanHong
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500144
Subject(s) - chromatography , cassia , emodin , chemistry , micellar electrokinetic chromatography , anthraquinones , capillary electrophoresis , sodium , organic chemistry , botany , medicine , alternative medicine , pathology , traditional chinese medicine , biology
An MEKC method was developed for the determination of the five pharmaceutically important anthraquinones: chrysophanol ( 1 ), physcion ( 2 ), emodin ( 3 ), aloe‐emodinin ( 4 ), and rhein (5) in Cassia obtusifolia (Leguminosae). A buffer solution (pH 9.00) composed of 20 mM sodium borate, 20 mM sodium deoxycholate (DOC), and 15% ACN was found to be the most suitable electrolyte for this separation. Regression equations revealed linear relationships (correlation coefficients: 0.9993, 0.9992, 0.9996, 0.9989, and 0.9991) between the peak area of each compound ( 1 , 2 , 3 , 4 , and 5 ) and its concentration. The RSDs of migration times and peak areas were <1.23 and 2.72% within 1 day, respectively. The effects of pH value, surfactant (DOC) concentration, and organic modifier on the migration were also studied. By this way, the contents of five anthraquinones in the extracts of the seed of C. obtusifolia (Leguminosae) from different sources were successfully determined within 14 min.