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Extraction and analysis of different Cannabis samples by headspace solid‐phase microextraction combined with gas chromatography‐mass spectrometry
Author(s) -
Ilias Yara,
Rudaz Serge,
Mathieu Patrick,
Christen Philippe,
Veuthey JeanLuc
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500130
Subject(s) - solid phase microextraction , chromatography , injection port , mass spectrometry , extraction (chemistry) , chemistry , gas chromatography , gas chromatography–mass spectrometry , polydimethylsiloxane , analytical chemistry (journal) , materials science , organic chemistry , composite material
A headspace solid‐phase microextraction combined with GC‐MS method was developed for the extraction and analysis of cannabinoids from Cannabis samples. Different commercially available fibres were evaluated; polydimethylsiloxane 100 μm was selected as the most efficient one. In order to enhance sensitivity and reduce analysis time, the sampling temperature was studied and it showed that extraction should be performed at a high temperature (150°C). In relation with the high lipophilicity of cannabinoids, a relatively long desorption time (3 min) was necessary to ensure a total transfer from the fibre into the injection port of the gas chromatograph. The method was finally applied to the extraction of Swiss marijuana samples from different regions. Data treatment by principal component analysis and hierarchical cluster analysis allowed a discrimination of the different batches.