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Determination of 12 pueraria components by high‐performance liquid chromatography‐mass spectrometry
Author(s) -
Lin ChingChe,
Wu ChuanI.,
Sheu ShuennJyi
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500126
Subject(s) - chemistry , chromatography , pueraria , isoflavones , puerarin , mass spectrometry , radix (gastropod) , glycosidic bond , high performance liquid chromatography , detection limit , liquid chromatography–mass spectrometry , alternative medicine , pathology , biology , enzyme , medicine , biochemistry , botany
Puerariae radix, a commonly used Chinese herb drug derived from the dried root of legume plant, contains a series of isoflavones as its chief pharmacologically active constituents. Using 12 pueraria components as markers, an LC‐UV‐MS method requiring less than 60 min, was developed for estimating the quality of pueraria samples within 60 min. Extracts were analyzed using a Cosmosil 5C 18 ‐MS column, by gradient elution with an aqueous solution of acetic acid and methanol–ACN at a flow‐rate of 1.0 mL/min. Peaks were detected at 254 nm and each peak was identified by LC/MS. The reproducibilities (RSD) of this proposed method, on the basis of peak‐area ratios from six replicate injections, were 0.93–1.42% (intraday) and 1.05–1.63% (interday) at a detection limit of 0.12–0.78 μg/mL. Most of the markers used in this study can be classified, respectively, into three major categories, namely, isoflavones, O ‐glycosidic isoflavones, and C ‐glycosidic isoflavones. The structures of the compounds were determined from LC‐MS fragmentation data and data from the literature.