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HPTLC‐densitometric method of determination of oleanolic acid in the Lamii albi flos
Author(s) -
WójciakKosior Magdalena,
Krzaczek Tadeusz,
Matysik Grazyna,
Skalska Agnieszka
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500106
Subject(s) - oleanolic acid , flos , chemistry , chromatography , absorbance , acetone , biochemistry , medicine , alternative medicine , rutin , pathology , antioxidant
HPTLC combined with densitometric method was used to determine the content of oleanolic acid in acetone extract from the Lamii albi flos L. The plant extract was separated on Si 60 HPTLC plates and determined with the use of two densitometric methods: measurement of fluorescence and absorbance. The content of oleanolic acid was statistically calculated. In a measurement of absorbance in 1 g of the dry Lamii albi flos L. 1458 μg of oleanolic acid was determined (0.147%). In a fluorescence method in 1 g of the plant material 1516 μg of oleanolic acid was determined (0.149%).