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Ion‐pair mediated transport of angiotensin, neurotensin, and their metabolites in liquid phase microextraction under acidic conditions
Author(s) -
Reubsaet J. Léon E.,
Loftheim Håvard,
Gjelstad Astrid
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500092
Subject(s) - chemistry , heptane , chromatography , extraction (chemistry) , neurotensin , acceptor , phase (matter) , solid phase extraction , organic chemistry , biochemistry , physics , receptor , neuropeptide , condensed matter physics
This paper discusses the behaviour of angiotensin 1 and neurotensin together with their metabolites in a three‐phase liquid phase microextraction under acidic conditions. Variations in donor phase, organic phase, and acceptor phase are studied with extraction recovery as response variable. It is proved that for all peptides the transport across the organic phase is mediated by heptane‐1‐sulphonic acid. n ‐Octanol gave overall best results as organic phase. A donor phase volume of 1.0 mL was chosen as a compromise between optimal recovery and robustness of the LPME device. The optimal pH of the donor phase (using acceptor phase of pH 2) was found to be different for the peptides, which opens opportunities for selective sample preparation. Decreasing the acceptor phase pH to 1.0 resulted in increased extraction recoveries. On using 1.0 mL of donor phase containing 50 mM heptane‐1‐sulphonic acid pH 3, n ‐octanol as organic phase immobilized in the pores of the fibre, and 20 μL of acceptor phase containing 0.1 mol/L HCl, extraction recoveries up to 82% (enrichment factor = 41) were achieved. To our knowledge this is the first report on liquid phase microextraction of angiotensins and neurotensins.