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Online trace enrichment to determine pyrethroids in river water by HPLC with column switching and photochemical induced fluorescence detection
Author(s) -
Martínez Galera Maria,
Barranco Martínez Dolores,
Parrilla Vázquez Piedad,
Gil García Maria Dolores
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500086
Subject(s) - chromatography , chemistry , derivatization , elution , detection limit , matrix (chemical analysis) , high performance liquid chromatography , fluorescence , pesticide , analytical chemistry (journal) , physics , quantum mechanics , agronomy , biology
The potential of online trace enrichment on a highly apolar short column in LC was evaluated for the determination of pyrethroids in river water. Twelve millilitres of water samples, modified with 8 mL ACN (ACN/water 40 : 60, v/v), were passed through 50×4.6 mm ID first separation column packed with 5 μm Hypersil Elite C 18 . Pesticides were preconcentrated in this column while the matrix background was eluted to waste. Separation of pesticides was performed on a 3.5 μm symmetric C 18 column (250×4.6 mm ID) with an ACN step gradient as mobile phase and fluorescence detection was used after postcolumn derivatization by using UV light. The use of photochemically induced fluorescence for detection improved sensitivity and selectivity. Quantification limits ranged from 0.05 to 0.1 μg/L and pesticide recoveries at two concentration levels (0.1 and 0.5 μg/L) were between 93.1 and 118.6%, with RSD between 2.5 and 7.5% ( n = 3) in river water samples. No matrix effect was detected.