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Simultaneous determination of cefepime and L‐arginine by micellar electrokinetic chromato‐ graphy and applications to commercial injections
Author(s) -
Chen YiRu,
Lin ShunJin,
Chou YuWei,
Wu HsinLung,
Chen SuHwei
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500081
Subject(s) - cefepime , chromatography , micellar electrokinetic chromatography , chemistry , tris , sodium dodecyl sulfate , ammonium acetate , detection limit , capillary electrophoresis , high performance liquid chromatography , biochemistry , antibiotic resistance , imipenem , antibiotics
A simple micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous analysis of cefepime and L‐arginine. The determination of cefepime and L‐arginine in pharmaceutical preparations was performed at 25°C using a background electrolyte consisting of Tris buffer with sodium dodecyl sulfate (SDS) as the electrolyte solution. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and SDS. Under optimal MEKC conditions, good separation with high efficiency and short analysis times is achieved. Using cefazolin as an internal standard, the linear ranges of the method for the determination of cefepime and L‐arginine were over 5–100 μg/mL; the detection limits of cefepime (signal to noise ratio = 3; injection 3.45 kPa, 3 s) and L‐arginine (signal to noise ratio = 3; injection 3.45 kPa, 3 s) were 2 μg/mL and 4 μg/mL, respectively. Applicability of the proposed method for the determination of cefepime and L‐arginine in commercial injections was demonstrated.