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Utilization of zirconia stationary phase as a tool in drug control
Author(s) -
Kučera Radim,
Žižkovský Václav,
Sochor Jaroslav,
Klimeš Jiří,
Dohnal Jiří
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500076
Subject(s) - cubic zirconia , drug , phase (matter) , stationary phase , chemistry , drug control , chromatography , computer science , pharmacology , medicine , organic chemistry , ceramic
Zirconia‐based stationary phases represent an interesting alternative to silica‐based materials. Two zirconia‐based stationary phases were studied as an option for use in drug analysis. The different properties of zirconia material, distinct from RP silica‐columns, were employed for the development of a novel and rapid stability monitoring HPLC method. This method enables simultaneous control of possible degradation processes of active substance (ibuprofen) as well as antimicrobial excipients (methyl‐ and propylparaben). The separation of ibuprofen, its two main degradation products 2‐(4‐isobutyrylphenyl)propionic acid and 4‐isobutylacetophenone, parabens, and 4‐hydroxybenzoic acid as their degradation product was successfully accomplished on a Zr‐CarbonC18 column using a mobile phase consisting of acetonitrile‐phosphate buffer (pH 4.8)‐propan‐2‐ol (27 : 56:17, v/v/v ). Detection was performed at 258 nm and the analysis was completed within 17 minutes.