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Detection system for electro‐separation analytical methods
Author(s) -
Štohl Radek,
Glovinová Eliška,
Pospíchal Jan
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500073
Subject(s) - scanner , computer science , process (computing) , capillary electrophoresis , controller (irrigation) , control system , process control , separation (statistics) , chromatography , control engineering , artificial intelligence , engineering , chemistry , machine learning , electrical engineering , agronomy , biology , operating system
The paper provides information about the on‐line monitoring of components analysed by capillary electrophoresis. For this purposes we developed a whole‐capillary transverse scanning detection system, which helps to improve and control the separation processes. A picture from a colour line scanner was used as a source of basic information for autonomous control of the separation process by regulation of the high voltage source. The application and algorithms for machine vision were designed in the progressive graphic development system LabVIEW. Real‐time control of the separation process was implemented in a compact control process logic controller. The performance of the detection system was evaluated and the function of the overall system was tested by performing isotachophoretic analysis of a model mixture.