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Estimation of partition coefficients by MEKC Part 2: Anthocyanins
Author(s) -
Müller Lukáš,
Bednář Petr,
Barták Petr,
Lemr Karel,
Ševčík Juraj
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500072
Subject(s) - partition (number theory) , partition coefficient , chromatography , chemistry , mathematics , combinatorics
Abstract The octanol‐water partition coefficients of seven anthocyanins (in neutral form, pH = 7.0) were measured by means of MEKC. A new iterative method for the estimation of migration time of electroosmotic flow (EOF) and micelles in MEKC is presented. This calculation is based on Gauss‐Newton linearization of the dependence between migration indices and migration times of a set of suitable standards and the application of an advanced statistical evaluation procedure. The values of partition coefficients obtained with the aid of an iterative process are compared with values obtained on use of EOF and micelle markers.