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Dynamic ultrasound‐assisted extraction of polyphenols in tobacco
Author(s) -
Gu Xungang,
Cai Jibao,
Zhu Xiaolan,
Su Qingde
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500064
Subject(s) - chromatography , polyphenol , extraction (chemistry) , chlorogenic acid , rutin , chemistry , ascorbic acid , solvent , quercitrin , scopoletin , analyte , sample preparation , organic chemistry , medicine , alternative medicine , food science , pathology , antioxidant
A novel extraction method, namely dynamic ultrasound‐assisted extraction, is investigated. This technique is efficient with respect to both time and solvent consumption because it utilizes ultrasonic energy in dynamic mode during extraction. Polyphenols (chlorogenic acid, esculetin, rutin, scopoletin, and quercitrin) are extracted from a tobacco ( Nicotina tobaccum L.) sample for 10 min with 6 mL of solvent. Fresh solvent is continuously pumped through the sample, with which the analytes can be rapidly extracted, and the possibility of degradation efficiently avoided. Methanol involving 0.5% w/v ascorbic acid was used as extraction solvent; optimal flow rate and extraction time were investigated. The extract was cleaned up by C 18 disposable cartridge. The spiked and nonspiked tobacco samples were used for the evaluation of the proposed method. Recoveries obtained were varied from 96 to 108% and RSDs from 2.0 to 4.6%. This extraction technique was revealed to recover larger amounts of polyphenols from tobacco, compared to the static ultrasound‐assisted extraction method.