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Determination of vitamin B 5 in human urine by high‐performance liquid chromatography coupled with mass spectrometry
Author(s) -
Heudi Olivier,
Fontannaz Patric
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500048
Subject(s) - chromatography , urine , chemistry , mass spectrometry , detection limit , calibration curve , quantitative analysis (chemistry) , coefficient of variation , high performance liquid chromatography , vitamin , liquid chromatography–mass spectrometry , biochemistry
In the present work, we have developed a simple and rapid liquid chromatography/mass spectrometry (LC/MS) method for the identification and quantification of vitamin B 5 in human urine. Urine was spiked with vitamin B 5 internal standard, hopantenic acid (HOPA), and then diluted with the LC mobile phase prior to its analysis by LC/MS. The quantification was performed in single ion monitoring mode. The calibration curve was linear ( r 2 = 0.999) between 0.25 to 10 μg/mL. With a limit of detection of 0.1 μg/mL the method was sensitive enough to determine low levels of vitamin B 5 in urine. The overall quantitative efficiency of the method was evaluated by spiking urine samples with four different concentrations of vitamin B 5 ; the intra‐assay coefficient of variation was below 5% and the recoveries were between 96 to 108%. The results of the present study show that the proposed method is selective and sensitive enough for the quantification of vitamin B 5 in urine.