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Capillary electrochromatography using polyelectrolyte multilayer coatings
Author(s) -
Kamande Mary W.,
Fletcher Kristin A.,
Lowry Mark,
Warner Isiah M.
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500006
Subject(s) - capillary electrochromatography , electrochromatography , polyelectrolyte , capillary electrophoresis , electrokinetic phenomena , micellar electrokinetic chromatography , chromatography , analyte , biomolecule , capillary action , electro osmosis , materials science , chemistry , adsorption , electrophoresis , nanotechnology , polymer , organic chemistry , composite material
This review covers recent progress in polyelectrolyte multilayer (PEM) coatings applied to analytical separations using open‐tubular capillary electrochromatography (OT‐CEC). The simple preparation procedure involved in the PEM approach has provided some attractive features over other modes of capillary electrophoresis‐based separations including packed column capillary electrochromatography (PC‐CEC) and micellar electrokinetic chromatography (MEKC). PEM coatings have been used to alleviate the adsorption of basic analytes, to improve separations, and to improve the stability of the electroosmotic flow. Fundamental aspects of PEM coatings on surfaces and analytical separation platforms are briefly outlined in this review. In addition, applications of PEM coatings to fused‐silica capillaries or microchip separation devices for the separation of small achiral or chiral analytes, as well as large biomolecules, are discussed.