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Supercritical fluid chromatography tandem‐column method development in pharmaceutical sciences for a mixture of four stereoisomers
Author(s) -
Barnhart Wesley W.,
Gahm Kyung H.,
Thomas Sam,
Notari Steve,
Semin David,
Cheetham Janet
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200500005
Subject(s) - supercritical fluid chromatography , chromatography , chemistry , tandem , elution , resolution (logic) , supercritical fluid , high performance liquid chromatography , organic chemistry , materials science , artificial intelligence , computer science , composite material
A tandem‐column method using Chiralpak AD‐H and Chiralcel OD‐H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i. e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% ethanol:isopropanol (50 : 50 v/v ). On the contrary, this mixture (mixture A) could not be baseline separated by SFC conditions explored with individual Chiralpak AD‐H and Chiralcel OD‐H columns. The effects of various mobile phases on elution order, capacity factor, selectivity, and resolution were determined with mixture A on the individual aforementioned columns to develop the tandem‐column method.