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Modification of a poly(methyl methacrylate) injection‐molded microchip and its use for high performance analysis of DNA
Author(s) -
Zhou XiaoMian,
Dai ZhongPeng,
Liu Xin,
Luo Yong,
Wang Hui,
Lin BingCheng
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401920
Subject(s) - methyl methacrylate , methacrylate , dna , materials science , poly(methyl methacrylate) , chromatography , chemistry , polymer , polymerization , composite material , biochemistry
Inexpensive and permanently modified poly(methyl methacrylate)(PMMA) microchips were fabricated by an injection‐molding process. A novel sealing method for plastic microchips at room temperature was introduced. Run‐to‐run and chip‐to‐chip reproducibility was good, with relative standard deviation values between 1–3% for the run‐to‐run and less than 2.1% for the chip‐to‐chip comparisons. Acrylonitrile‐butadiene‐styrene (ABS) was used as an additive in PMMA substrates. The proportions of PMMA and ABS were optimized. ABS may be considered as a modifier, which obviously improved some characteristics of the microchip, such as the hydrophilicity and the electro‐osmotic flow (EOF). The detection limit of Rhodamine 6G dye for the modified microchip on the home‐made microchip analyzer showed a dramatic 100‐fold improvement over that for the unmodified PMMA chip. A detection limit of the order of 10 –20 mole has been achieved for each injected φX‐174/HaeIII DNA fragment with the baseline separation between 271 and 281 bp, and fast separation of 11 DNA restriction fragments within 180 seconds. Analysis of a PCR product from the tobacco ACT gene was performed on the modified microchip as an application example.