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Rapid determination of C 6 ‐aldehydes in tomato plant emission by gas chromatography–mass spectrometry and solid‐phase microextraction with on‐fiber derivatization
Author(s) -
Deng Chunhui,
Li Ning,
Zhu Weimin,
Qian Ji,
Yang Xiaofeng,
Zhang Xiangmin
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401912
Subject(s) - solid phase microextraction , derivatization , chromatography , mass spectrometry , chemistry , gas chromatography–mass spectrometry , gas chromatography , fiber , organic chemistry
Abstract A simple, rapid, sensitive, and solvent‐free method was developed for determination of plant‐signalling compounds, the three C 6 ‐aldehydes hexanal, ( Z )‐3‐hexenal, and ( E )‐2‐hexenal, in tomato plant emission by gas chromatography–mass spectrometry (GC‐MS) and solid‐phase microextraction (SPME) with on‐fiber derivatization. In this method, O ‐2,3,4,5,6‐(pentafluorobenzyl)hydroxylamine (PFBHA) in aqueous solution was first headspace adsorbed onto a 65 μm poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber at 25°C for 5 min, and then the fiber with adsorbed PFBHA was used for headspace extraction of tomato plant emission at 25°C for 6 min. Finally, the resulting oximes adsorbed on the fiber were desorbed and analyzed by GC‐MS. Extraction conditions and method validation were studied. The proposed method had low detection limit values for the three aldehydes from 0.1 to 0.5 ng/L and good precision (RSD less than 10%). In this work, the method was applied to investigation of tomato plant defense response to Helicoverpa armigera.

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