Premium
Capillary columns in liquid chromatography: between conventional columns and microchips
Author(s) -
Saito Yoshihiro,
Jinno Kiyokatsu,
Greibrokk Tyge
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401902
Subject(s) - chromatography , capillary action , electrospray , chemistry , mass spectrometry , high performance liquid chromatography , sample preparation , column (typography) , analytical chemistry (journal) , materials science , computer science , telecommunications , frame (networking) , composite material
Liquid chromatography on columns with small internal diameters has been reviewed as the intermediate technique between conventional liquid chromatography and microchip separations. The development of micro column separations in the early years has been described, starting with the papers of Horváth and co‐workers and Ishii and co‐workers, continuing into the first part of the eighties, then making a leap in time to recent innovations with small‐bore columns. Based on internal diameters a classification of the different analytical HPLC columns has been suggested. The advantages of small‐bore columns have been discussed, with particular emphasis on the advantage of coupling to concentration sensitive detectors when the sample amount is limited. Open tubular columns are treated as a part of the historic background. The recent developments include a brief look into the current status of monolithic columns, the use of packed nano columns and micro columns with electrospray mass spectrometry, and the potential of two‐dimensional comprehensive liquid chromatography. Finally, the coupling of sample preparation to analytical columns and the future applications of the novel technological improvements to the microchip separation methods have been discussed.