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Enantiomer separations on a vancomycin stationary phase and retention mechanism of pressurized capillary electrochromatography
Author(s) -
Yao Chuanyi,
Tang Shaokun,
Gao Ruyu,
Jiang Chun,
Yan Chao
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401875
Subject(s) - capillary electrochromatography , chemistry , electrochromatography , enantiomer , chromatography , capillary electrophoresis , capillary action , electro osmosis , promethazine , electrophoresis , analytical chemistry (journal) , materials science , anesthesia , organic chemistry , composite material , medicine
Several chiral drugs, promethazine, carteolol, celiprolol, and albuterol, were resolved with vancomycin as the chiral stationary phase by pressurized capillary electrochromatography (pressurized CEC) and capillary HPLC. The effects of pressure and electrical field strength on efficiency, resolution, and capacity factor in pressurized CEC were investigated. A mathematical model describing the relationship of capacity factor in pressurized CEC with voltage, pressurized flow velocity, electroosmotic mobility, and electrophoretic mobility was established, which was in good agreement with the experimental data.