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Wheat digalactosyldiacylglycerol molecular species profiling using porous graphitic carbon stationary phase
Author(s) -
Deschamps Frantz S.,
Gaudin Karen,
Baillet Arlette,
Chaminade Pierre
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401874
Subject(s) - chemistry , alkyl , formic acid , tetrahydrofuran , triethylamine , elution , acetonitrile , toluene , chromatography , partition coefficient , methanol , chloroform , fatty acid methyl ester , organic chemistry , catalysis , solvent , biodiesel
The potential of porous graphitic carbon stationary phase (PGC) was assessed for the separation of molecular species of digalactosyldiacylglycerol (DGDG). Detection was by an evaporative light scattering detector (ELSD). A conventional optimization strategy allowed definition of a quaternary non‐aqueous mobile phase and separation of 9 wheat DGDG molecular species with isocratic elution: methanol/toluene/tetrahydrofuran/chloroform 64.3/21.5/13.7/0.5 v/v with 0.1% of triethylamine and a stoichiometric amount of formic acid. The molecular species were identified by LC/MS. The chromatographic behavior of DGDG on PGC was then compared to previous studies. The addition of a carbon double bond on the alkyl chain decreased the retention. This contribution was less important when the number of unsaturations increased in the alkyl chain. The consequence of this retention behavior with PGC was an elution order of molecular species which did not agree with the partition number as observed with C18 grafted stationary phases.