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Preparation and characterization of C 16 monolithic columns for capillary electrochromatography
Author(s) -
Zhang Kai,
Yan Chao,
Yang Junjiao,
Zhang Zhichao,
Wang Qinsun,
Gao Ruyu
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401873
Subject(s) - methacrylamide , capillary electrochromatography , monolithic hplc column , monomer , electrochromatography , chromatography , homologous series , polymerization , porosity , chemistry , reproducibility , materials science , analytical chemistry (journal) , high performance liquid chromatography , capillary electrophoresis , polymer , organic chemistry , acrylamide
A series of methacrylamide‐based C 16 monolithic columns were prepared and characterized to determine how their porous structural properties and chromatographic behavior are affected by the percentages of functional monomer, base monomer, and cross‐linker in the polymerization solution. Baseline separation of 6 neutral compounds can be readily obtained in an optimized column. Furthermore, the effects of organic additive in the mobile phase, operating voltages, and temperature on retention behaviors and separation efficiencies were also studied. The separation mechanism is also discussed. High column efficiency and good reproducibility indicate that the monolithic columns hold considerable promise.