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Application of programmable temperature vaporisation injection with resistive heating‐gas chromatography flame photometric detection for the determination of organophosphorus pesticides
Author(s) -
Patel Katan,
Fussell Richard J.,
Goodall David M.,
Keely Brendan J.
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401872
Subject(s) - chemistry , chromatography , gas chromatography , omethoate , methamidophos , pesticide , ethyl acetate , injector , acephate , agronomy , biology , engineering , mechanical engineering
The combination of a programmable temperature vaporisation (PTV) injector with resistive heating GC (RH‐GC), a form of fast GC, has been applied to the analysis of organophosphorus (OP) pesticides. The PTV injector was optimised in the ‘at‐once’ solvent vent mode for the injection of ethyl acetate (10–40 μL) or ACN (10 μL). The short RH‐GC column (5 m×0.25 mm ID) with fast temperature ramps (up to 153°C/min) allowed the separation of a total of 20 OP pesticides in less than 6 min. Average recoveries between 67 and 119% were obtained for pesticides spiked at 0.01 mg/kg into apple and pear matrix. Extraction of orange juice with ACN provided higher recoveries (92–104%) for methamidophos, acephate and omethoate compared to ethyl acetate (62–73%). Results for analysis of OP pesticides in samples containing incurred residues were in good agreement with those obtained using GC‐MS. The overall method was rapid, allowing 20 samples to be analysed in 4 h. British Crown Copyright © 2005 Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim