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Separation, pre‐concentration, and HPLC analysis of methylxanthines in urine samples
Author(s) -
Zydroń Mirosława,
Baranowski Jacek,
Baranowska Irena
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401841
Subject(s) - theobromine , theophylline , chemistry , chromatography , urine , high performance liquid chromatography , caffeine , bronchodilator , paraxanthine , acetonitrile , pharmacology , asthma , biochemistry , medicine , metabolism , cytochrome p450 , cyp1a2
An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1‐methylxanthine, 3‐methylxanthine, 7‐methylxanthine, 1‐methyluric acid, 1,3‐dimethyluric acid, 1,7‐dimethyluric acid, and 1,3,7‐trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP‐18 (7 μm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.