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LC‐PDA and LC‐ESI‐MS separation and determination of process‐related substances arising from stilbene‐type fluorescent whitening agents. Application to monitoring of their photodegradation products in industrial effluents and aqueous environmental systems
Author(s) -
Rao R. Nageswara,
Venkateswarlu N.,
Khalid Sara,
Narsimha R.
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401839
Subject(s) - photodegradation , chemistry , aqueous solution , fluorescence , chromatography , effluent , electrospray , industrial effluent , mass spectrometry , organic chemistry , photocatalysis , catalysis , environmental science , physics , quantum mechanics , environmental engineering
A simple and rapid gradient elution high‐performance liquid chromatographic method using photodiode array and electrospray ionization mass spectrometric detectors was developed for separation and determination of the process‐related substances and photodegradation products of stilbenesulfonic acids, viz. 4,4´‐dinitrostilbene‐2,2´‐disulfonic acid (DNSDA), 4‐amino‐4´‐nitrostilbene‐2,2´‐disulfonic acid (ANSDA), and 4,4´‐diaminostilbene‐2,2´‐disulfonic acid (DASDA) in industrial waste waters. Gradient elution was carried out using ammonium acetate and acetonitrile as mobile phase and an Inertsil‐ODS 3V column for separation. The negative‐ion electrospray ionization mass spectra containing [M–H] – ions of sulfonic acids allowed molecular mass determination of unknowns and the structures were proposed on the basis of the fragment ions in the MS/MS spectra.