Premium
Characterization of polyacrylamide based monolithic columns
Author(s) -
Plieva Fatima M.,
Andersson Jonatan,
Galaev Igor Yu.,
Mattiasson Bo
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401836
Subject(s) - polyacrylamide , acrylamide , copolymer , monolithic hplc column , monomer , materials science , ether , chromatography , chemistry , polymer chemistry , chemical engineering , polymer , high performance liquid chromatography , organic chemistry , composite material , engineering
Supermacroporous monolithic polyacrylamide (pAAm)‐based columns have been prepared by radical cryo‐copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co‐monomer, allyl glycidyl ether (AGE), and cross‐linker N,N ´‐methylene‐bis‐acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge‐like structure with interconnected supermacropores of pore size 5–100 μm. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column‐to‐column reproducibility of pAAm‐monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same cryostructuration conditions.