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Chiral separation of omeprazole and several related benzimidazoles using supercritical fluid chromatography
Author(s) -
del Nozal M. Jesús,
Toribio Laura,
Bernal José L.,
Alonso Cristina,
Jiménez Juan J.
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401835
Subject(s) - supercritical fluid chromatography , chemistry , chromatography , supercritical fluid , enantiomer , high performance liquid chromatography , organic chemistry
A study of the enantiomeric separation of omeprazole and several related benzimidazoles, using supercritical fluid chromatography (SFC), on the amylose based column Chiralpak AD is presented in this work. The effect of the organic modifier as well as temperature on the retention and enantioresolution was investigated. Alcohol‐type modifiers provided the best results, allowing the enantiomeric separation of all the compounds studied with resolutions that were in most cases higher than 2, and analysis times lower than 10 minutes. An investigation of the temperature effect revealed that the isoelution temperature was below the working temperature range in only two cases, and hence it was better to work at the highest temperature permitted.