Premium
Peak shape improvement of basic analytes in capillary liquid chromatography
Author(s) -
Prüß Anja,
Kempter Christine,
Gysler Jens,
MaierRosenkranz Jürgen,
Jira Thomas
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401807
Subject(s) - capillary action , chromatography , chemistry , analyte , capillary electrochromatography , impurity , capillary electrophoresis , analytical chemistry (journal) , materials science , organic chemistry , composite material
The analysis of bases is of special interest in pharmaceutical research because numerous active substances contain basic functional groups. Capillary and conventional size LC separations of drug substances spiked with potential impurities were compared. In the case of the nonpolar drug levonorgestrel equal separation efficiency was readily attained by both techniques. The peaks of basic substances, however, showed extensive tailing when separated by capillary LC. The peak deformation was attributable to interactions of the basic substances with the polar inner surface of the fused silica capillaries employed in capillary LC and does not appear with the steel tubing generally used in conventional size LC. This drawback of capillary LC was overcome by use of deactivated fused silica capillaries for column hardware and transfer lines.