Premium
On the potential of electrochemically modulated liquid chromatography of proteins in a micro open parallel plate separator
Author(s) -
LapizcoEncinas Blanca H.,
Pinto Neville G.
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401771
Subject(s) - separator (oil production) , chromatography , chemistry , theoretical plate , analytical chemistry (journal) , high performance liquid chromatography , stationary phase , physics , thermodynamics
Electrochemically Modulated Liquid Chromatography (EMLC) is a technique in which an electrical potential is applied to the stationary phase in order to alter its adsorptive properties. The micro Open Parallel Plate Separator (μOPPS) is an open‐channel micro‐chromatographic system with the unique characteristic of an adjustable rectangular flow cross‐section. In this publication, the performance of the μOPPS in the EMLC mode was simulated using a comprehensive mathematical model. The separation of two proteins was analyzed by performing a parametric study for the effects of the potential applied to the stationary phase, the dimensions of the device, and the operating conditions. It is shown that the combination of the μOPPS and EMLC is promising for protein separations. These separations can be optimized by carefully selecting the applied potential and the geometry of the μOPPS. The marriage of the μOPPS and EMLC adds two additional dimensions for control, to improve the separation efficiency in analytical applications and in preparative separations of trace compounds.