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Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection
Author(s) -
Bérangère Claude,
Caussarieu Nathalie,
Morin Philippe,
MorinAllory Luc,
Lafosse Michel
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401764
Subject(s) - chemistry , ursolic acid , betulinic acid , chromatography , oleanolic acid , selectivity , porosity , carbon fibers , light scattering , organic chemistry , scattering , materials science , physics , pathology , composite number , optics , medicine , genetics , alternative medicine , composite material , biology , catalysis
Abstract An original system which uses Porous Graphitic Carbon as support and a mixture of organic solvents as mobile phase is proposed for the analysis of triterpenic acids by liquid chromatography. The separation of betulinic acid, ursolic acid, oleanolic acid, and 18α‐ and 18β‐glycyrrhetinic acids was carried out within a short time and monitored by evaporative light scattering detection as universal detection method. Molecular modelling studies show that the main contribution to the selectivity comes from the electrostatic interaction characterised by the dipole moment of the products.