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Liquid chromatography analysis of monosubstituted sulfobutyl ether‐β‐cyclodextrin isomers on porous graphitic carbon
Author(s) -
Jacquet Romain,
Pennanec Rodolphe,
Elfakir Claire,
Lafosse Michel
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401758
Subject(s) - chemistry , cyclodextrin , acetonitrile , ether , chromatography , ammonium acetate , enantiomer , phase (matter) , high performance liquid chromatography , organic chemistry
The retention behaviour of the three positional isomers of monosubstituted sulfobutyl ether‐β‐cyclodextrin was investigated on a porous graphitic carbon (PGC) column. The influence of the mobile phase composition (nature and concentration of organic and electronic modifiers) was studied as well as the effect of column temperature. These hydrophilic and anionic analytes were highly retained on the PGC stationary phase compared to octadecyl bonded phases. The retention is mainly governed by a reversed‐phase mechanism with electronic interaction playing a secondary role. An increase in solute retention and efficiency with temperature was observed. Successful isocratic separation with satisfactory baseline resolution of the three isomers of monosubstituted sulfobutyl ether‐β‐cyclodextrin was achieved at 75°C on a Hypercarb column by using ammonium acetate as electronic modifier in water‐acetonitrile (83 : 17). The chromatographic methodology developed can be easily used for relative quantification of each isomer within a mixture and can be applied for semi‐preparative purification of each one. The evaporative light scattering detector allows the detection of these non UV‐visible absorbing molecules.