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Resolution of triacylglycerol positional isomers by reversed‐phase high‐performance liquid chromatography
Author(s) -
Momchilova Svetlana,
Tsuji Koichiro,
Itabashi Yutaka,
NikolovaDamyanova Boryana,
Kuksis Arnis
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200401746
Subject(s) - chromatography , chemistry , high performance liquid chromatography , reversed phase chromatography , phase (matter) , resolution (logic) , hydrophilic interaction chromatography , structural isomer , organic chemistry , computer science , artificial intelligence
The ability of reversed‐phase high‐performance liquid chromatography (RP‐HPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethanol, 2‐propanol, 1‐propanol, 1‐butanol, acetone, or dichloromethane were tested for the separation of POP‐PPO, PLP‐PPL, PEP‐PPE, and PDP‐PPD (P – palmitic, O – oleic, L – linoleic, E – eicosapentaenoic, D – docosahexaenoic acid residue) on a single RP‐HPLC column. The resolution improved with increasing number of double bonds in the acyl residues. While POP and PPO were only partially resolved, PDP and PPD were fully separated with all tested mobile phases, except those containing methanol. Also separated were the four TAGs having the same equivalent carbon number (ECN = 42), PEP, PPE, PDP, and PPD, on a single RP‐HPLC column with mobile phase acetonitrile – 2‐propanol (70:30, v/v ) at 0.8 mL/min. In all cases the isomer with the unsaturated acyl residue in either 1‐ or 3‐position was retained more strongly than the respective 2‐isomer.