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Use of inorganic salts during the etching process in the fabrication of chemically modified capillaries for open tubular electrochromatography
Author(s) -
Pesek Joseph J.,
Matyska Maria T.,
Velpula Sunandini
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400100
Subject(s) - silanization , capillary electrochromatography , electrochromatography , chemistry , etching (microfabrication) , fabrication , salt (chemistry) , monolith , electrophoresis , chemical engineering , moiety , chromatography , analytical chemistry (journal) , capillary electrophoresis , organic chemistry , medicine , alternative medicine , layer (electronics) , pathology , engineering , catalysis
Capillaries for use in electrophoretic analyses are etched with ammonium bifluoride and in some cases a second inorganic salt is included in the process. The effects of the presence of these inorganic components in the surface matrix on the electromigration of heterocyclic aromatic amines and enkephalins are evaluated. Resolution, efficiency, and peak shape are used to compare the various columns. In one instance, the etched surface is then modified by the addition of an octadecyl moiety using a silanization/hydrosilation procedure. The surface properties of the various etched capillaries are also compared by electroosmotic flow measurements.