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The determination of sulfonamides in honey by high performance liquid chromatography – mass spectrometry method (LC/MS)
Author(s) -
Krivohlavek Adela,
Šmit Zdenko,
Baštinac Martina,
Žuntar Irena,
PlavšcPlavšic Franjo
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400084
Subject(s) - chromatography , american foulbrood , detection limit , repeatability , solid phase extraction , chemistry , analyte , mass spectrometry , extraction (chemistry) , honey bee , biology , ecology
The sulfonamides (SAs) are stable chemotherapeutics used against the bacterial disease affecting bees, known as American foulbrood (Bacillus larvae), so their residues could appear in the honey of treated bees. Their presence at a concentration above the limit value could be a potential danger to human health. Therefore, a simple, rapid, and reliable method for determination of 11 available SAs in honey was optimized. The samples were homogenized and cleaned with extraction on solid phase by means of Chromabond C 18 end‐capped cartridge followed by LC/MS analyses. A detection limit of 25 μg/kg was achieved for all analytes. The repeatability of the method was proven and the optimal parameters for temperature and pH of the mobile phase and acetic buffer, respectively, were determined. In this study, 20 samples of domestic honey were included. Six of the analyzed samples were positive, but all results were below the Croatian permissible limit value (100 μg/kg).