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Capillary electrophoretic determination of triamterene, methotrexate, and creatinine in human urine
Author(s) -
Rodríguez Flores Juana,
Berzas Nevado Juan J.,
Durán Merás Isabel,
Rodríguez Gómez Maria J.
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400059
Subject(s) - capillary electrophoresis , chromatography , chemistry , triamterene , triethanolamine , urine , phosphoric acid , creatinine , electrophoresis , isotachophoresis , electrolyte , analytical chemistry (journal) , hydrochlorothiazide , biochemistry , medicine , organic chemistry , electrode , blood pressure , radiology
A capillary zone electrophoresis (CZE) method using a fused‐silica capillary (60.2 cm×75 μm ID) was investigated for the determination of triamterene (TRI), methotrexate (MTX), and creatinine (CREA) in human urine. The separation was performed using a hydrodynamic injection time of 7 s (0.5 psi), a voltage of 25 kV, a capillary temperature of 30°C, and 40 mM phosphoric acid adjusted to pH 2.25 by addition of triethanolamine as separation electrolyte. Under these conditions, analysis takes about 15 min. A linear response over the 0.5–15.0 mg L –1 concentration range was found for TRI and MTX, and 0.5–80.0 mg L –1 for CREA. Dilution of the sample (water:urine, 1 : 1 for TRI and MTX, and 1:25 for CREA determination) was the only step necessary prior to analysis by electrophoresis. The developed method is easy, rapid, and sensitive and has been applied to determine triamterene, methotrexate, and creatinine in urine samples with satisfactory results.