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Studies on thermionic ionisation detection in comprehensive two‐dimensional gas chromatography
Author(s) -
Ryan Danielle,
Marriott Philip
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400033
Subject(s) - gas chromatography , flame ionization detector , analytical chemistry (journal) , two dimensional gas , chemistry , detection limit , detector , chromatography , analyte , nitrogen , physics , optics , organic chemistry
This study explores the application of specific thermionic ionisation detection in comprehensive 2‐D GC (GC×GC) and represents the first report of GC×GC with nitrogen phosphorus detection (GC×GC‐NPD). Of particular interest is the performance of the NPD with respect to peak parameters of asymmetry and sensitivity. Since GC×GC produces much narrower peaks than obtained with fast GC ( e. g. 100 ms vs. < 1 s) the effect of detector response time and any lack of symmetry arising from the detection step is important if peak separation (resolution) is to be maintained. It was observed that detector gas flows had a significant impact on peak asymmetry and peak magnitude, and that optimisation of the detector was critical, particularly for complex sample analysis by GC×GC‐NPD. Peak asymmetries ranging from A s = 1.8 to 8.0 were observed under different conditions of detector gas flows. Comparison of GC×GC‐NPD with GC×GC‐flame ionisation detection (FID) showed the former to be approximately 20 times more sensitive for the detection of nitrogen‐containing methoxypyrazines analytes, and GC×GC‐NPD had a larger linear detection range compared to GC×GC‐FID. Furthermore, comparison of GC×GC‐NPD and GC×GC‐TOFMS chromatograms for the analysis of coffee headspace demonstrated the benefits of selective detection, ultimately realised in a comparatively simplified contour plot.