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Analysis of isomeric tropane alkaloids from Schizanthus grahamii by very fast gas chromatography
Author(s) -
Bieri Stefan,
Muñoz Orlando,
Veuthey JeanLuc,
Christen Philippe
Publication year - 2006
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400008
Subject(s) - tropane , chemistry , gas chromatography , chromatography , stereochemistry
This study presents a very fast GC analysis applied for the baseline separation of isomeric tropane alkaloids extracted from the stem‐bark of Schizanthus grahamii (Solanaceae). The work provided a challenging application where isothermal analysis in conjunction with very short narrow bore columns (3 m×100 μm ID and 1.5 m×50 μm ID) was particularly suited for the speeding up. Experimental parameters were used in the optimisation steps, including selection of stationary phase, temperature, internal column diameter and optimal practicable gas velocity. Some considerations about sample injection in fast isothermal analysis are also briefly presented. Finally, the investigated approach allowed a very fast baseline separation of four positional and configurational isomers in less than 9 s.