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Development of a HPTLC method for in‐process purity testing of pentoxifylline
Author(s) -
Grozdanovic Olivera,
Antic Dusan,
Agbaba Danica
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400006
Subject(s) - chromatography , chemistry , pentoxifylline , chloroform , acetone , detection limit , toluene , impurity , organic chemistry , medicine , pharmacology
A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by‐products as impurities, LiChrospher® RP‐18 F254s chromatoplates, acetone‐chloroform‐toluene‐dioxane (2:2:1:1 v/v ) as a mobile phase, and detection at 275 nm were employed. Linearity ( r ⪈ 0.997), recovery (86.5–115.5%), and determination limit (0.1–0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline‐containing raw materials and pharmaceuticals.