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Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high‐speed counter‐current chromatography
Author(s) -
Li HuaBin,
Chen Feng
Publication year - 2005
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200400002
Subject(s) - chromatography , chemistry , ethyl acetate , countercurrent chromatography , extraction (chemistry) , ethanol , hexane , umbelliferone , elution , imperatorin , high performance liquid chromatography , traditional medicine , coumarin , organic chemistry , medicine
Cnidium monnieri (L.) Cusson is a well‐known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin‐related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high‐speed counter‐current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High‐speed counter‐current chromatography with the two‐phase solvent systems n ‐hexane–ethyl acetate–ethanol–water (5 : 5 : 4 : 6, v/v ) and n ‐hexane–ethyl acetate–ethanol–water (5 : 5 : 6 : 4, v/v ) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6–98.9%, and the recoveries were 85.7–94.2%.