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Determination of biogenic amine neurotransmitters by RP‐HPLC/FL method on Chromolith RP18e stationary phase
Author(s) -
Baranowska Irena,
Zydron Miroslawa
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390084
Subject(s) - chemistry , chromatography , methanol , high performance liquid chromatography , amine gas treating , acetonitrile , reversed phase chromatography , phosphate buffered saline , buffer (optical fiber) , phase (matter) , analytical chemistry (journal) , organic chemistry , telecommunications , computer science
The Chromolith RP‐18e stationary phase (Merck, Darmstadt, Germany) was tested for the separation of biogenic amine neurotransmitters and their derivatives. Citrate, acetate, and phosphate buffer solutions with methanol and acetonitrile as modifiers were used as the mobile phases. The tested column proved its high efficiency for the separation of the compounds of interest, resulting in short retention times and permitting working with low pressure. Three gradient systems were developed. The best results were obtained for acetate buffer solution with methanol as the modifier. The system was applied to the analysis of urine samples.