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Capillary electrochromatographic separation of ionizable compounds with a molecular imprinted monolithic cationic exchange column
Author(s) -
Yan Weiying,
Gao Ruyu,
Zhang Zhichao,
Wang Qinsun,
Jiang Chun Ver,
Yan Chao
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390076
Subject(s) - capillary electrochromatography , electrochromatography , chemistry , capillary electrophoresis , cationic polymerization , ionic strength , chromatography , monolithic hplc column , capillary action , molecular imprinting , buffer (optical fiber) , polymerization , electrophoresis , polymer , high performance liquid chromatography , selectivity , materials science , polymer chemistry , organic chemistry , aqueous solution , telecommunications , computer science , composite material , catalysis
A polymer‐based monolithic capillary column imprinted with 4‐aminopyridine (4‐AP) was prepared by a thermally‐initiated polymerization process; and its performance as a capillary electrochromatographic medium was evaluated in separating 4‐AP and 2‐AP isomers. The effects of experimental parameters, such as pH value and ionic strength of the buffer, the acetonitrile content in the mobile phase, and the applied voltage, on the resolution of these isomers had been carefully investigated. It was found that in the retention process there were interplays of multiple mechanisms of ion‐exchange, molecular imprinting, and electrophoresis. These mechanisms allowed more sophisticated control of experimental parameters in the separation of ionizable compounds.