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Analysis of gastrodin and tetramethylpyrazine in traditional Chinese preparations by micellar electrokinetic chromatography
Author(s) -
Rongying Wang,
Wanyun Ma,
Dieyan Chen
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390074
Subject(s) - tetramethylpyrazine , gastrodin , chromatography , chemistry , micellar electrokinetic chromatography , detection limit , analyte , capillary electrophoresis , medicine , alternative medicine , pathology
A simple, rapid, and accurate micellar electrokinetic chromatographic method has been developed for determination of gastrodin and tetramethylpyrazine in three traditional Chinese preparations: Zhennaoning jiaonang , Yangxue shengfa jiaonang , and Xiaoshuan zaizao wan . Running buffer comprising 50 mM sodium tetraborate and 15 M sodium dodecylsulfate (SDS), pH 9.50, was found to be most suitable for the separation. All experiments was performed with a 47 cm (40 cm effective length)×75 μm ID uncoated fused‐silica capillary and UV detection at 200 nm. The linear calibration ranges were 2.5–200 μg mL –1 ( R = 0.999) for gastrodin and 5.0–200 μg mL –1 ( R = 0.997) for tetramethylpyrazine; the detection limits were 0.5 μg mL –1 and 0.8 μg mL –1 , respectively. Recoveries of the two analytes from the samples, calculated by use of a method described in detail in the text, were between 94.21 and 104.46%. The amounts of gastrodin and tetramethylpyrazine in the preparations were easily determined within 10 min.